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By using a novel, robust, fully-automated approach, we have shown:
- The density of activated microglia is a very sensitive measure of disease state in the α-synuclein PFF mouse model
- The phenotypic state of the microglia can be more finely quantified using:
- The distribution of activation scores, which can disentangle effects from the increase in the cell density and the changes in their morphology
- The distribution of activated microglia across different subclasses with distinct morphology
This approach may provide sensitive measures for the preclinical assessment of the efficacy of putative disease-modifying therapeutic agents in this mouse model of Parkinson's disease.