Multiplex Immunofluorescence (IF)
Multiplex immunofluorescence (IF) represents a cutting-edge technique in preclinical research focused on neurodegenerative diseases, enabling simultaneous detection and visualization of multiple proteins within neural tissues with high precision. By leveraging a combination of fluorescently-labeled antibodies and/or reporter systems, multiplex immunofluorescence allows for the evaluation of numerous biomarkers and protein interactions concurrently, offering a comprehensive understanding of complex molecular changes characteristic of CNS diseases.
This advanced method facilitates the exploration of intricate protein networks, cellular pathways, and spatial relationships among various molecular targets, empowering a deeper comprehension of disease pathogenesis and the identification of potential therapeutic targets crucial for developing effective interventions and treatments for neurodegenerative diseases.
At Biospective, our lab is equipped with multiple IHC autostainers that are capable of efficient multiplex IF staining. Our team has developed multiplex IF protocols for our CNS disease rodent models. Our laboratory R&D team is continually developing new protocols, and we can work with you to define optimal staining for your study.
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How many stains can Biospective do on a single slide?
That really depends on the particular markers. There are several approaches, such as using primary antibodies from different species, or kits that allow for sequential staining with any combination of antibodies. We can scan up to ~8 channels on our digital slides scanners. There is also some dependency on the antigen retrieval pretreatments and conditions. We are currently developing "hi-plex" methods that will allow us to increase the number of stains per slide.
Can Biospective develop protocols for new markers?
Absolutely. Our R&D team focuses on exactly this type of work. We can optimize conditions (e.g. antigen retrieval, dilutions, staining sequence) for optimal staining.
When would multiplex IF vs. standard IHC be performed?
It really depends on what we are trying to evaluate. Single markers or markers with special antigen retrieval conditions generally work well with standard IHC. When we are looking for relationships between multiple markers (e.g. co-expression), then multiplexing is ideal.