Regional Distribution of Activated Microglia in PFF Mice

Table of Contents

Abstract

Presentation

01. Introduction 02. Microglial Classification 03. Machine Learning Model 04. Activation Score 05. Alpha-Synuclein Mouse Model 06. Microglia Density 07. Microglia Activation 08. Activation vs. Density 09. Mouse Model Activation Score 10. Clustering 11. Summary

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Classification of activated microglia (red boxes) in the hippocampus at 3 months post-injection of PBS (top left) and human α-synuclein PFFs (top right) into the anterior olfactory nucleus.

In the low magnification view (bottom), we can readily appreciate the quantity and distribution of activated microglia (red boxes) in the ipsilateral and contralateral hemispheres of the PFF-injected mice at the level of the entorhinal cortex and posterior hippocampus.

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This figure shows the classification of activated, Iba-1 positive microglia in the PBS- and PFF-injected mouse brains at 3 months post-injection. Microglia are typically non-activated and sparsely distributed in the brains of PBS control mice. In contrast, we can readily observe that the microglia are densely and extensively distributed, and often activated, in the brains of the PFF-injected mice.