Spatiotemporal Relationship between Amyloid & Inflammation

Table of Contents

Abstract

Presentation

01. Introduction 02. Neuroinflammation Classification 03. Microglial Activation 04. Plaque Microenvironment 05. AD Mouse Model 06. Age & Amyloid 07. Age & Neuroinflammation 08. Age & Microglial Activation 09. Microenvironment Analysis 10. Age & Inflammatory Microenvironment 11. Summary

A complex spatiotemporal relationship exists between amyloid-β & neuroinflammation in Alzheimer's disease

https://opt003stagmediafiles.blob.core.windows.net/image/2871bfe2060646b6970540556bd787dc

Multiplex immunofluorescence (IF) demonstrating Iba-1 positive microglia and GFAP positive astrocytes intimately associated with β-amyloid plaques, as well as distributed outside of the local neighborhood of these plaques.

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Improving our understanding of the complex spatial relationships between β-amyloid plaques, activated microglia, and reactive astrocytes may facilitate the development of disease-modifying therapeutics for Alzheimer's disease. The ability to generate quantitative metrics associated with the "inflammatory microenvironment" of β-amyloid pathology is an essential component of this spatial biology approach.

In this presentation, we describe our high-throughput approach to interrogate the inflammatory microenvironment of β-amyloid pathology. We have applied this novel method to an APP/PS1 transgenic mouse model of Alzheimer's disease that is well-suited to the preclinical assessment of experimental therapeutics agents.