A complex spatiotemporal relationship exists between amyloid-β & neuroinflammation in Alzheimer's disease
Multiplex immunofluorescence (IF) demonstrating Iba-1 positive microglia and GFAP positive astrocytes intimately associated with β-amyloid plaques, as well as distributed outside of the local neighborhood of these plaques.
Improving our understanding of the complex spatial relationships between β-amyloid plaques, activated microglia, and reactive astrocytes may facilitate the development of disease-modifying therapeutics for Alzheimer's disease. The ability to generate quantitative metrics associated with the "inflammatory microenvironment" of β-amyloid pathology is an essential component of this spatial biology approach.
In this presentation, we describe our high-throughput approach to interrogate the inflammatory microenvironment of β-amyloid pathology. We have applied this novel method to an APP/PS1 transgenic mouse model of Alzheimer's disease that is well-suited to the preclinical assessment of experimental therapeutics agents.