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Examples of concentric radial ROIs derived from the β-amyloid plaque segmentation. The yellow contour lines illustrate the boundaries of the radial ROIs. All pixels on a given line are equidistant to the edge of a plaque. Scale bars are 10µm.
Iba-1 stain density (A), microglia density (B), activated microglia density (C; inset shows full y-axis scale), and GFAP stain density (D) in the cerebral cortex of ARTE10 mouse model at 9 months-of-age as a function of the distance to plaques. The dashed lines indicate the levels of each metric in wild-type (WT) animals. Statistical analysis was performed using Kruskal-Wallis non-parametric test and Dunn’s multiple comparisons test. *p<0.05; **p<0.005; ***p<0.001; ****p<0.0001.