Age-dependent Neuroinflammation in APP/PS1 Mouse Model

Table of Contents

Abstract

Presentation

01. Introduction 02. Neuroinflammation Classification 03. Microglial Activation 04. Plaque Microenvironment 05. AD Mouse Model 06. Age & Amyloid 07. Age & Neuroinflammation 08. Age & Microglial Activation 09. Microenvironment Analysis 10. Age & Inflammatory Microenvironment 11. Summary

https://opt003stagmediafiles.blob.core.windows.net/image/7f3487ffbe0243ebba58a4bc79422de1

Representative snapshots of the neuroinflammatory microenvironment of β-amyloid plaques at various time points in the anterior cerebral cortex. Activated and non-activated microglia are indicated by white and orange boxes, respectively. Scale bars are 100µm.

https://opt003stagmediafiles.blob.core.windows.net/image/17c5c62f3d6f4a648b926e51cd484d87

Quantitative data showing age-dependent increase of Iba-1 stain density (A), microglia density (B), activated microglia density (C), and GFAP stain density (D) in the cerebral cortex of ARTE10 mouse model. N=10-12 mice per group. Statistical analysis was performed using Kruskal-Wallis non-parametric test and Dunn’s multiple comparisons test. *p<0.05; **p<0.005; ***p<0.001; ****p<0.0001.

--:--

We have quantified markers of microgliosis (Iba-1 stain density, total microglia, and activated microglia density), as well as astrogliosis (GFAP stain density) in the cerebral cortex of APP/PS1 mice at different time points. The transgenic mice demonstrated progressive, age-dependent increase in all of these measures.