Age-dependent Beta-Amyloid Pathology in APP/PS1 Mice

Table of Contents

Abstract

Presentation

01. Introduction 02. Neuroinflammation Classification 03. Microglial Activation 04. Plaque Microenvironment 05. AD Mouse Model 06. Age & Amyloid 07. Age & Neuroinflammation 08. Age & Microglial Activation 09. Microenvironment Analysis 10. Age & Inflammatory Microenvironment 11. Summary

https://opt003stagmediafiles.blob.core.windows.net/image/9b7c6c3f25cb4f9d9fc0999b7f5e7926

Representative snapshots of segmented β-amyloid plaques at various time points in the anterior cerebral cortex. The plaques are shown in red and the nuclei are shown in blue. The plaques identified by our algorithm are indicated by the white boxes.

https://opt003stagmediafiles.blob.core.windows.net/image/89cf8d5396be42d6b9afef3bc7f344c4

Quantitative data showing age-dependent increase in the density of β-amyloid plaques in the cerebral cortex of ARTE10 mouse model. N=10-12 mice per group. Statistical analysis was performed using Kruskal-Wallis non-parametric test and Dunn’s multiple comparisons test. *p<0.05; **p<0.005; ***p<0.001; ****p<0.0001.

--:--

We have applied our automated plaque segmentation algorithm to tissue sections from transgenic mice at 6, 9, and 12 months-of-age, as well as 6 month-old age-matched wild-type (WT) mice as a reference. Consistent with the literature on this APP/PS1 model, we observe an age-dependent accumulation of β-amyloid in the cerebral cortex and other brain regions.