Representative snapshots of segmented β-amyloid plaques at various time points in the anterior cerebral cortex. The plaques are shown in red and the nuclei are shown in blue. The plaques identified by our algorithm are indicated by the white boxes.
Quantitative data showing age-dependent increase in the density of β-amyloid plaques in the cerebral cortex of ARTE10 mouse model. N=10-12 mice per group. Statistical analysis was performed using Kruskal-Wallis non-parametric test and Dunn’s multiple comparisons test. *p<0.05; **p<0.005; ***p<0.001; ****p<0.0001.
We have applied our automated plaque segmentation algorithm to tissue sections from transgenic mice at 6, 9, and 12 months-of-age, as well as 6 month-old age-matched wild-type (WT) mice as a reference. Consistent with the literature on this APP/PS1 model, we observe an age-dependent accumulation of β-amyloid in the cerebral cortex and other brain regions.