Biospective has extensive expertise performing studies in brain and spinal cord with a broad spectrum of stains. One of our particular strengths is quantitative image analysis of digitized histology/IHC slides using our unique PERMITS™ software. All studies are performed in-house with strict attention to quality control.
| Perfusion fixation and tissue dissection |
| Tissue embedding & processing |
| FFPE & frozen tissue sectioning |
| Histological & IHC staining |
| Whole slide brightfield and fluorescence digitization |
| Histology |
|---|
| H&E |
| Cresyl violet |
| Luxol Fast Blue (LFB) |
| Perl’s Iron/Prussian Blue |
| IHC |
|---|
| Beta-amyloid |
| Tau |
| Alpha-synuclein |
| TDP-43 |
| GFAP (astrocytes) |
| IBA-1 (macrophages/microglia) |
| NeuN (neurons) |
| Neurofilament light chain (axons) |
| Synaptophysin (synapses) |
| MBP, PLP (myelin) |
| APP/SMI-32 (axonal damage) |
| IgG/Fibrinogen (vasogenic edema) |
| Inflammatory cell markers |
| Collagen IV (vessels) |
| Histology |
|---|
| H&E |
| Cresyl violet |
| Luxol Fast Blue (LFB) |
| Perl’s Iron/Prussian Blue |
| Histology |
|---|
| Histology |
| H&E |
| Cresyl violet |
| Luxol Fast Blue (LFB) |
| Perl’s Iron/Prussian Blue |
| IHC |
|---|
| Beta-amyloid |
| Tau |
| Alpha-synuclein |
| TDP-43 |
| GFAP (astrocytes) |
| IBA-1 (macrophages/microglia) |
| NeuN (neurons) |
| Neurofilament (axons) |
| Synaptophysin (synapses) |
| MBP, PLP (myelin) |
| APP/SMI-32 (axonal damage) |
| IgG/Fibrinogen (vasogenic edema) |
| Inflammatory cell markers |
| Collagen IV (vessels) |
| IHC |
|---|
| IHC |
| Beta-amyloid |
| Tau |
| Alpha-synuclein |
| TDP-43 |
| GFAP (astrocytes) |
| IBA-1 (macrophages/microglia) |
| NeuN (neurons) |
| Neurofilament light chain (axons) |
| Synaptophysin (synapses) |
| MBP, PLP (myelin) |
| APP/SMI-32 (axonal damage) |
| IgG/Fibrinogen (vasogenic edema) |
| Inflammatory cell markers |
| Collagen IV (vessels) |
PERMITS™ is Biospective’s proprietary and automated software. It has been developed to provide accurate quantification of immunohistochemistry data from digitized tissue sections. It can analyze individual sections from a broad range of tissues, and includes accurate 3D reconstruction of brain tissue sections for correlation of in vivo imaging and post-mortem histology/IHC data from mouse or rat brain. Contact us about Customized Applications.
| Misfolded protein (alpha-synuclein, TDP-43, tau, beta-amyloid) burden |
| Myelin quantification |
| Neuronal density |
| Neuroinflammation |
| Vascular density |
| MRI-IHC co-registration (3D PERMITS™) |
PERMITS™ is Biospective’s proprietary and automated software. It has been developed to provide accurate quantification of immunohistochemistry data from digitized tissue sections. It can analyze individual sections from a broad range of tissues, and includes accurate 3D reconstruction of brain tissue sections for correlation of in vivo imaging and post-mortem histology/IHC data from mouse or rat brain. Contact us about Customized Applications.
| Misfolded protein (alpha-synuclein, TDP-43, tau, beta-amyloid) burden |
| Myelin quantification |
| Neuronal density |
| Neuroinflammation |
| Vascular density |
| MRI-IHC co-registration (3D PERMITS™) |
| Misfolded protein (alpha-synuclein, TDP-43, tau, beta-amyloid) burden |
| Myelin quantification |
| Neuronal density |
| Neuroinflammation |
| Vascular density |
| MRI-IHC co-registration (3D PERMITS™) |
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