Biospective’s in vivo PD models rely on the inoculation of synthetic α-synuclein fibrils to initiate Parkinson’s disease-like Lewy bodies/Lewy neurites and transmit the pathology to anatomically-connected regions. These transgenic mice and α-synuclein fibril spreading models can mimic early “prodromal stage” or late “motor stage” of PD. Common injection sites include the striatum, the cerebral cortex, the substantia nigra, and the anterior olfactory nucleus (AON).
When synuclein is inoculated into the AON, the model shows neurodegeneration (which can be monitored longitudinally using in vivo MRI and neurofilament biochemistry), as well as progressive changes in olfaction and sleep. This model also shows neuronal loss and significant neuroinflammation by quantitative IHC.